Monkey Mischief!: Party Time is a collection of 20 mini-games for up to four players. Four monkeys have escaped from their prison, using a banana to unlock their cell door, and now they cause mischief in a village. Each game only lasts one minute, so different games can be played quickly in succession. The titles are divided into four groups and have to be unlocked gradually. Many games and assets are very identical to the earlier Block Party by the same developer, but now with a monkey theme.

In Crowd Control the monkey needs to pump the nunchuck like a water pump to spray water at bird that appears from the back of the screen. In Crazy Bucket Rhino the monkeys descend from a swing and can hop down to collect food and goodies. They need to collect as much as possible and then head back up, but when they leave the swing they are exposed to a rampaging rhino that can cause the monkey to lose everything that was collected. In another game players swing a rhino by its tail like in the hammer throw sport to launch the rhino over water in a specific direction that needs to be timed to collect items. In Monkey Masterpiece players need to trace a shape with the Wii Remote. If they fail to follow the shape a monkey sitting in the back will scream.

Monkey Dodgeball is a game of dodgeball played in teams of two monkeys with multiple balls thrown at once. In another game players shoot toilet rolls from a first-person perspective towards monkeys appearing from and dancing on toilets. There is also a mini-game called Monkey Memory based on the cups and balls magic routine where players have to guess where a ball is, moving quickly between and under three cups. If they guess wrong, a monkey in the back is electrocuted. In a pogo game the monkeys bounce in a small circle and need to knock each other out. In Salmon Slap players use the nunchuck's analog stick to move around a circular arena. They then swing the Wii Remote to hit an opponent with a fish.