The game features both single-player as well as two-player co-operative multiplayer as players traverse through a series of levels in a style similar to Super Mario 64. The game features six playable characters (Ratchet, Clank, Jak, Daxter, Sly Cooper, and Bentley). It also features a total of 50 levels, which can be categorized into five "archetypes", five weapon classes and four environments namely Paris from Sly Cooper & Bentley, Metropolis City on Kerwan from Ratchet & Clank, Haven City from Jak and Daxter and Planet X which is an original environment created for the game. Players can choose which character they want to play as on each level, each character will have a different gameplay style and may have advantages on certain levels. Co-operative play allows the second player to play as the first player's sidekick character for example if the first player selects Jak as his character, the second player will automatically be assigned Daxter.

The five weapon classes are mainly Disc, Whip, Bowling, Projectile and Melee. The disc is thrown in a similar fashion to a frisbee, once thrown players navigate it around the level remotely using the Move controller to achieve objectives for example to hit moving targets. The whip is controlled by the Move controller via 1 to 1 movement. Bowling acts similar to the disc where the player throws the bowling ball and subsequently guides it with the Move controller. Projectiles involve the use of mainly guns as well as other weapons such as grenade launchers. Melee uses each character's iconic weapons such as Sly's cane; Ratchet's wrench or Jak's gunstaff to hit enemies in a fashion similar to their original games.