Mario is invited to Peach's castle, but when he arrives Peach is nowhere to be seen. He soon learns from Toad that Bowser has once again kidnapped her. The Koopa King has also put a spell on her castle, imprisoning her subjects. So it is up to Mario to break the spell and rescue Peach.

Super Mario 64 is the first Mario game done entirely with 3D graphics. The core gameplay is similar to previous platform installments, focusing on jumping, avoiding obstacles, and defeating enemies. Levels can be explored without time limits. Stars must be collected in order to unlock new areas, eventually gaining access to various parts of Bowser's castle. Defeating Bowser on each floor procures keys necessary to unlock big doors and enter other floors, which contain more areas.